MiFish Pipeline

Analyzing Fish eDNA amplified by MiFish primers.

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MiFish is a set of universal PCR primers for metabarcoding environmental DNA (eDNA) that are shed into waters from fishes. MiFish primers target a hypervariable region of fish mitochondrial 12S rRNA gene (approximately 160-190 bp), which contains information to identify fishes to taxonomic family, genus and species except for some closely related congeners. After amplification by MiFish primers, MiFish pipeline accepts your sequence data in FASTQ (paired-end or single file) or FASTA format and returns a species list.

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